Journal: Cell death discovery
Article Title: CeDaD-a novel assay for simultaneous tracking of cell death and division in a single population.
doi: 10.1038/s41420-025-02370-7
Figure Lengend Snippet: Fig. 2 Cell division and cell death rates can be analyzed from one cell sample. HCT116 wt cells were treated for 2 days with AMG 232, YKL- 5-124, or volasertib. Representative CellTrace Violet staining of DMSO control and 10−6 M volasertib-treated cells was gated by number of cell divisions within 2 days (A). Cell division distribution was compared between both conditions (B, n = 3). Based on the cell division analysis and following the appropriate equation (C) cell division rates were calculated as average cell divisions per day (D). For cell death analysis, cell populations were gated by Apotracker Green and propidium iodide (PI) staining. In representative gating analyses of DMSO control (E) and 10−6 M Volasertib-treated cells (F) were gated as viable, apoptotic, dead, or artifact. Cell death rates represented by apoptotic and dead cell populations were compared between treatments (G, n = 3). Cell division rates (H) and cell death rates (I) of the DMSO control and after volasertib treatment (10−6 M and 10−7.5 M) were compared between HCT116 wt and LIN37−/−/RB−/−cells. Mean ± SD are given, and P values were calculated by two-way ANOVA (*P <0.05; **P <0.01; ***P <0.001).
Article Snippet: Cell death and division assay For combined analysis of cell division and cell death via flow cytometry, cells were washed once with PBS and stained with 5 μM CellTrace Violet (Thermo Fisher) in 1ml PBS based on a 6.6mM CellTrace Violet solution in DMSO for 30min.
Techniques: Staining, Control